Mass Spectrometry

The BYU Chemistry Department supports two mass spectrometry facilities. Our biological mass spectrometry facility provides top-tier data acquisition and sample analysis in proteomics, metabolomics, lipidomics, and other omics. Our general mass spectrometry facility provides high resolution identification and analysis across several applications

Common Experiment Types

Proteomics; identification and quantification
Lipidomics; identification, quantification, collision cross-section analysis
Small Molecule; identification, quantification, collision cross-section analysis

Instruments

Agilent Ion Mobility LC/Q-ToF

Thermo Orbitrap Fusion Lumos

Thermo Orbitrap Eclipse

Agilent LC/Q-TOF and TOF

Agilent 6560 Ion Mobility LC/Q-ToF

The Agilent 6560 Ion Mobility LC/Q-ToF (Agilent) is located in C021 BNSN.

IMS Control Document

The Agilent combines chromatography, ion mobility, and mass spectrometry to deliver superior separation and selectivity in analyses. Featuring high-accuracy separation capacity, impressive separation of isobaric classes, thorough characterizations of structures and compounds, and a low-energy drift tube that conserves the structure of gas molecules, the Agilent boosts BYU lab operations.

Policies and Procedures

Use of the Agilent costs $30/hour. All times are rounded up to the nearest 5-minute increment for analyses lasting 60 minutes or less and to the nearest 10 minute increment for analyses longer than 60 minutes.
All sample analyses must be performed by a lab technician.
Samples will not be analyzed unless they are accompanied by a fully completed checklist, which can be downloaded here.
Samples should be prepared according to the methods listed below.
1. On-Filter Trypsin Digest
2. In Gel Sample Prep for LCMS Sequencing
If data acquired and/or analyzed by the BYU Agilent is included in a publication, financial support from the Fritz B. Burns Foundation should be acknowledged in the publication. For example: “Mass spectrometry was supported by the Fritz B. Burns Foundation (2016 gift to Brigham Young University).”

Orbitrap Fusion Lumos Tribrid Mass Spectrometer

The Orbitrap Fusion Lumos Tribrid Mass Spectrometer (Orbitrap) is located in C021 BNSN.

The Orbitrap excels at low-level PTM analyses, multiplexed relative quantitation using isobaric tags, intact protein characterization, and MSⁿ analysis of small molecules. Equipped with bright ion sources, a segmented quadrupole mass filter, advanced vacuum technology, a high-capacity ETD fragmenter, the Advanced Peak Determination (ADP) algorithm for data-dependent experiments, and ultra-high resolution lasers for isobaric compound analysis, the Orbitrap allows for state-of-the-art mass spectrometry.

Policies and Procedures

Use of the Orbitrap costs $30/hour. All times are rounded up to the nearest 5-minute increment for analyses lasting 60 minutes or less and to the nearest 10 minute increment for analyses longer than 60 minutes.
All sample analyses must be performed by a lab technician.
Samples will not be analyzed unless they are accompanied by a fully completed checklist, which can be downloaded here.
Samples should be prepared according to the methods listed below.
1. On-Filter Trypsin Digest
2. In Gel Sample Prep for LCMS Sequencing
If data acquired and/or analyzed by the BYU Orbitrap is included in a publication, financial support from the Fritz B. Burns Foundation should be acknowledged in the publication. For example: “Mass spectrometry was supported by the Fritz B. Burns Foundation (2016 gift to Brigham Young University).”

Orbitrap Eclipse Tribrid Mass Spectrometer

Agilent LC/QTOF and TOF

MASS SPECTROMETRY