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Chemistry and Biochemistry Labs
Chemistry and Biochemistry Labs

Mass Spectrometry Machines

Thermo Orbitrap Astral:

  • The Thermo Scientific™ Orbitrap™ Astral™ mass spectrometer, coupled to a Vanquish™ Neo LC system, delivers exceptional speed, sensitivity, and depth for discovery proteomics. Its Astral analyzer enables very high acquisition rates with high resolution and accurate mass, making it well suited to narrow-window data-independent acquisition (DIA) for deep, quantitative proteome coverage, as well as low-input and high-throughput workflows. Typical experiments use [gradient length — to confirm] gradients with approximately [load — to confirm] on column; methods are tailored in consultation with users to balance depth and throughput. The platform supports label-free and multiplexed (TMT) quantitation for both large-scale profiling and sensitive, sample-limited studies. [To be verified by facility staff.] 

Thermo Orbitrap Eclipse Tribrid: 

  • The Thermo Scientific™ Orbitrap Eclipse Tribrid™ mass spectrometer, coupled to an Ultimate 3000 LC system, is optimized for high-sensitivity proteomic and biological sample analysis. The system is configured with a trap-and-analytical column setup, providing exceptional sample cleanliness and reproducibility compared to direct-injection configurations. Typical gradients run 2 hours per sample, but methods can be adapted from 45 minutes to 4 hours depending on sample complexity and experimental needs. Standard sample loading is approximately 1 µg, though this can be adjusted in consultation with users to balance sensitivity and throughput.
  • A variety of acquisition methods are available to accommodate diverse research goals. Data-dependent acquisition (DDA) is routinely used for both MS2-based abundance measurements and MS1-based kinetic analyses requiring high-resolution isotope pattern quantification. The instrument also supports targeted MS2 and MS3 workflows, including TMT-based multiplexed quantitation and custom targeted assays. Data-independent acquisition (DIA) methods are available for comprehensive proteome coverage. Together, these options enable both discovery-based and hypothesis-driven studies with high accuracy, dynamic range, and flexibility.