Separations

High-Performance Liquid Chromatography (HPLC) is a powerful analytical technique used for the separation, identification, and quantification of individual components in a mixture. It involves pumping a liquid sample through a column packed with a stationary phase, where the components of the mixture interact differently with the stationary phase and are separated based on their affinity for the mobile and stationary phases, allowing for the analysis of a wide range of compounds, including pharmaceuticals, biomolecules, and environmental pollutants. Our system has both a UV-Vis diode array detector (DAD) and a Fluorescence detector (FLD). Furthermore, our system has a heated column compartment and an autosampler.

Gas Chromatography-Triple Quadrupole Mass Spectrometry (GC-MS/MS or GC-QqQ) is an advanced analytical technique that combines the separation capabilities of gas chromatography (GC) with the sensitivity and selectivity of a triple quadrupole mass spectrometer. It involves the separation of volatile and semi-volatile compounds in a gas chromatograph, followed by the selective isolation, fragmentation, and detection of targeted analytes using multiple reaction monitoring (MRM) in the triple quadrupole mass spectrometer, allowing for the highly specific and sensitive quantification of trace-level compounds in complex matrices.

The cost of using this instrument is $3/sample for internal BYU users and $10/sample for external. (Data analysis or sample prep will cost extra for external users).

Capillary Electrophoresis (CE) is an analytical technique used for the separation and analysis of charged molecules, such as proteins, peptides, and small ions, based on their differential migration in an electric field. The separation takes place in a narrow capillary filled with an electrolyte solution, where the analytes are separated according to their charge-to-size ratio, allowing for high-resolution separations with minimal sample consumption and fast analysis times. Our system has an auto sampler and a laser induced fluorescence (LIF) detector/source.

The Bruker Impact II GC-QTOF is a high-resolution gas chromatography–quadrupole time-of-flight mass spectrometer designed for advanced qualitative and quantitative analysis. It combines fast GC separation with accurate-mass QTOF detection to deliver exceptional sensitivity and mass accuracy. The system is well suited for non-targeted screening, trace-level identification, and complex mixture analysis. Its high resolving power enables confident compound identification through exact mass measurements and isotopic pattern analysis. We have automated liquid injection and the ability to manually inject head space and SPME.

The cost of using this instrument is $3/sample for internal BYU users and $10/sample for external. (Data analysis or sample prep will cost extra for external users).

The Shimadzu GC-2031AF FID/TCD is a versatile gas chromatograph designed for reliable routine and advanced gas analysis. Ours provides both flame ionization detection (FID) for sensitive organic compound analysis and thermal conductivity detection (TCD) for permanent gases and inorganic components. The system offers high stability, fast temperature programming, and excellent reproducibility for quantitative work. Its flexible configuration allows simultaneous or sequential use of multiple detectors to broaden application scope. Our instrument has an autosampler.

The cost of using this instrument is $3/sample for internal BYU users and $10/sample for external. (Data analysis or sample prep will cost extra for external users).