Separations
The BYU Spectroscopy facility is proud to host a variety of separations instruments. Please see below for a complete list.
Agilent 1260 HPLC

High-Performance Liquid Chromatography (HPLC) is a powerful analytical technique used for the separation, identification, and quantification of individual components in a mixture. It involves pumping a liquid sample through a column packed with a stationary phase, where the components of the mixture interact differently with the stationary phase and are separated based on their affinity for the mobile and stationary phases, allowing for the analysis of a wide range of compounds, including pharmaceuticals, biomolecules, and environmental pollutants. Our system has both a Uv-Vis diode array detector (DAD) and a Fluorescence detector (FLD). Furthermore, our system has a heated column compartment and an autosampler.
Agilent GC-MS/MS Triple Quad

Gas Chromatography-Triple Quadrupole Mass Spectrometry (GC-MS/MS or GC-QqQ) is an advanced analytical technique that combines the separation capabilities of gas chromatography (GC) with the sensitivity and selectivity of a triple quadrupole mass spectrometer. It involves the separation of volatile and semi-volatile compounds in a gas chromatograph, followed by the selective isolation, fragmentation, and detection of targeted analytes using multiple reaction monitoring (MRM) in the triple quadrupole mass spectrometer, allowing for the highly specific and sensitive quantification of trace-level compounds in complex matrices.
Sciex Capillary Electrophoresis

Capillary Electrophoresis (CE) is an analytical technique used for the separation and analysis of charged molecules, such as proteins, peptides, and small ions, based on their differential migration in an electric field. The separation takes place in a narrow capillary filled with an electrolyte solution, where the analytes are separated according to their charge-to-size ratio, allowing for high-resolution separations with minimal sample consumption and fast analysis times. Our system has an autosampler.